Antifungal activity of polymethyl methacrylate/Si3N4 composites against Candida albicans

Previous studies using gram-positive and -negative bacteria demonstrated that hydrolysis of silicon nitride (Si3N4) in aqueous suspensions elutes nitrogen produces gaseous ammonia while buffering pH. According to immunochemistry assays, fluorescence imaging, situ Raman spectroscopy, we demonstrate here the antipathogenic surface chemistry Si3N4 can be extended polymethylmethacrylate (PMMA) by compounding it with a minor fraction (~8 vol.%) particles without any tangible loss bulk properties. The hydrolytic products, which were eluted from partly exposed at composite surface, exhibited fungicidal action against Candida albicans. Using specific nitrative stress sensing dye highly resolved micrographs, observed congestion peroxynitrite (ONOO?) radicals mitochondria cells PMMA/Si3N4 composite, these absent identical monolithic PMMA. These observations suggest mimics antifungal activity macrophages, concurrently produce NO superoxide anions (O2•?) resulting formation candidacidal ONOO?. properties composites could used dental appliances inhibit uncontrolled growth albicans ensuing candidiasis being synergic chemoprophylaxis. In follow-up previous gram-negative bacteria, containing Hydrolytic products Highly microscopy revealed synergy